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. 2020 Oct 26;11:571300. doi: 10.3389/fimmu.2020.571300

FIGURE 3.

FIGURE 3

In-depth characterization of the heterogeneity within the CD4+ T cell compartment. (A) Heatmap showing the marker expression values for the 17 identified CD4+ T cell clusters within the T cell panel (32 samples; 818,800 cells). Cluster IDs and cluster frequencies are displayed at the bottom of the heatmap. (B) First-level HSNE embedding of the expression values of the indicated markers. (C) Boxplots of sample frequencies, divided per trimester, of the clusters plotted as a fraction of total CD4+ T cells. The Kruskal-Wallis with Dunn’s test for multiple comparisons was performed. (D) HSNE embedding of the expression values of the indicated markers, measured by flow cytometry and gated within CD3+CD4+ T cells. CD4+CD25+CD127- clusters are circled in orange; CD4+CD25+CD127+ clusters are circled in blue. 1st (n = 3), 2nd (n = 4) and term decidua (n = 4). (E) Boxplots depicting the CD25+CD127- (upper panel) and CD25+CD127+ (lower panel) populations as percentage of CD4+ T cells. (F) PCA of the sample frequencies (as percentage of total CD4+ T cells) where the gestational age groups are depicted along the first two components. The centroid of each group is indicated in gray. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.005.