Figure 1.
Characterization of bBMMSCs (magnification: ×40; bars: 100 μm). (A) The bBMMSCs at passage 3 (P3) exhibited fibroblastic morphologies with dendritic spindle shapes. (B) Positive expression of MSC-specific cell surface molecule (CD44) and absence of hematopoietic cell surface molecule (CD45) were identified in bBMMSCs by flow cytometry. The ratios were presented as mean%±standard error of the mean. (C) The bBMMSCs demonstrated differentiation potentials toward adipocytes (oil red staining) and osteoblasts (Von Kossa and alizarin red staining). bBMMSCs, bovine bone marrow-derived mesenchymal stem cells.