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. 2020 Jul 28;33(12):2021–2030. doi: 10.5713/ajas.20.0238

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Copyright © 2020 by Asian-Australasian Journal of Animal Sciences

This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Analysis of stable RGs by geNorm. (A) The ranking of stability (M values) of RGs in bBMMSCs is presented from the least stable RG (left side of the graph) to the most stable RGs (right side of the graph). (B) The optimal number of RGs (NF_opt) during normalization in bBMMSCs was recommended as 7 RGs (NF₇) by pairwise variation (V_7/8). (C) High correlation (r = 0.999, p<0.01) between NF₃ and NF₇ was identified by Pearson’s correlation analysis. RGs, reference genes; bBMMSCs, bovine bone marrow-derived mesenchymal stem cells; NF, normalization factor; V_n/n+1, pairwise variation between consecutively ranked NF (NF_n and NF_n+1); NF_opt, NF for optimal number of RGs; NF₇, NF_opt as 7 RGs; NF₃, NF for the three most stable RGs.