Table 1.
The primers used for gene cloning, quantitative real-time polymerase chain reaction
| Gene name | Accession number | Forward sequence (5′ to 3′) | Reverse sequence (5′ to 3′) |
|---|---|---|---|
| FABP4 | HQ833815.1 | CAAGCCCAATGTAACTATCA | ATCAAACTCTTCACCCAACT |
| SCD | HQ197924.1 | CTCGGTGGTGTTGCTGTGCT | GGTTCTCCCGTGGGTTGAT |
| FADS | XM013177620.1 | ATCTTTCAGTCTTCAGCGA | ACAGAGAGTGTTTTTCCCA |
| ELOVL1 | XM013177961.1 | TTTGGACCAGGGGGAA | GGAGATGTGGACAGAGACG |
| ELOVL5 | XM_013172275.1 | ACACCTACCTTTGTCTTCTC | TCAGCTTCACCTCCACT |
| ACAT2 | XM013186797.1 | GGAACTGGGACGGTAAC | GAGCCAAAGGCATAAGA |
| LPL | XM013188252.1 | TACCTGAAGACCCGTGC | ACTCTCATCTAAAGTGCCATAC |
| FASN | XM013197939.1 | AATCCAGAAGGGCCAAC | TCCAGCAATGCGGTAAG |
| RBP7 | XM_013194243.1 | TCGCAACACGCAAGATA | TCAAGCCAGTGAGTCCA |
| GAPDH | XM027449739.1 | GCCCAGAACATTATCCCA | CAGGTCAGGTCCACGACA |
| β-actin | M26111.1 | CGAGCGGTTCAGGTGTCCA | CGTCGTATTCCTGCTTGCTG |
FABP4, adipocyte fatty acid binding protein; SCD, stearoyl-CoA desaturase; FADS, fatty acid dehydrogenase; ELOVL1, elongase of long-chain fatty acid 1; ELOVL5, elongase of long-chain fatty acid 5; ACAT2, cholesterol acyltransferase 2; LPL, lipoprotein lipase; FASN, fatty acid synthase; RBP7, retinol binding protein 7; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; β-actin, beta-actin.