Table 2.
Primer sequences for real-time quantitative PCR analysis
| Genes | GenBank numbers | Primer sequences (5′→3′) | Product sizes (bp) |
|---|---|---|---|
| Mucin-1 | XM_015279046.2 | Forward: ACGCCTTCTTCAGCAGCAACTC Reverse: AGCAGCAGATGTGAGCAGTGATG |
183 |
| Mucin-2 | NM_001318434.1 | Forward: ACTGGACTTCACGGACACCT Reverse: CCCCCTCTACCATCATCAAA |
121 |
| Claudin-1 | NM_001013611.2 | Forward: GACCAGGTGAAGAAGATGCGGATG Reverse: CGAGCCACTCTGTTGCCATACC |
107 |
| occludin | XM_025144248.1 | Forward: CTGCTCTGCCTCATCTGCTTCTTC Reverse: CCATCCGCCACGTTCTTCACC |
143 |
| ZO-1 | XM_015278981.2 | Forward: GCCAGCCATCATTCTGACTCCAC Reverse: GTACTGAAGGAGCAGGAGGAGGAG |
172 |
| ZO-2 | NM_204918.1 | Forward: GAGAGCACAACCGAAGCAGAGG Reverse: TAGTCCTGTCCATAGCCACCATCC |
157 |
| Notch1 | NM_001030295.1 | Forward: GACAGCATCGCCGCCTTCAC Reverse: CGTCCAGGTTGATCTCGCAGTTG |
187 |
| Notch2 | NM_001252033.1 | Forward: CCAAAGCGAACCCTTGTTACC Reverse: CACACCCAAGAGAAGAATCAAGAG |
94 |
| DLL1 | NM_204973.2 | Forward: CTGGTCCTCATGCTGCTGCTG Reverse: CACCGATGACGCTGATGGAGATG |
166 |
| DLL4 | XM_421132.6 | Forward: GCTACTGTCCTTCTGGCTTCATGG Reverse: CATCGTCTCCAAGTCCTGCTGTG |
200 |
| Hes1 | XM_015291592.2 | Forward: GGACGCGCTGAAGAAGGATAGTTC Reverse: GCAGGTGCTTGACGGTCATCTC |
80 |
| Atoh1 | XM_004941130.3 | Forward: GGCTGAACCACGCCTTCGAC Reverse: GCGTCTCGTACTTGGAGAGCTTC |
81 |
ZO-1, zonula occludens-1; ZO-2, zonula occludens-2; DLL1, delta like canonical Notch ligand 1; DLL4, delta like canonical Notch ligand 4; Hes1, hes family bHLH transcription factor 1; Atoh1, atonal homolog 1.