Table 5.

Effect of corn resistant starch on the intestinal morphology of 21-day-old broilers

Items	Treatments1)					SEM	p-value
	NC	CS	4% RS	8% RS	12% RS		ANOVA	Linear2)	Quadratic2)
Duodenum
VH (μm)	839.60	840.12	800.98	836.23	813.06	14.97	0.893	0.709	0.625
CD (μm)	107.50a	107.08a	87.89b	89.58b	82.75b	2.56	<0.001	<0.001	0.118
VH/CD (μm/μm)	8.48	8.28	8.93	9.62	9.51	0.19	0.067	0.006	0.235
Jejunum
VH (μm)	705.68a	663.84c	681.17b	670.78bc	679.31bc	3.25	<0.001	0.166	0.443
CD (μm)	102.73b	101.15b	102.97b	102.74b	105.26a	0.35	<0.001	<0.001	0.594
VH/CD (μm/μm)	6.87a	6.56bc	6.62b	6.53cd	6.45d	0.03	<0.001	0.065	0.038
Ileum
VH (μm)	444.13ab	334.46c	378.85bc	435.16ab	478.90a	14.94	0.012	0.001	0.662
CD (μm)	98.07a	62.67c	62.90c	75.23bc	87.63ab	3.27	<0.001	<0.001	0.195
VH/CD (μm/μm)	5.12	5.68	6.39	6.05	5.82	0.21	0.425	0.750	0.544

Results are represented as the mean value±SEM (n = 8).

SEM, standard error of the mean; ANOVA, analysis of variance; VH, villus height; CD, crypt depth; VH/CD, the ratio of villus height to crypt depth.

¹⁾NC, a basic normal corn–soybean diet; CS, a corn–soybean–based diet supplementation with 20% corn starch (CS); 4% RS, 8% RS, and 12% RS, the corn–soybean–based diets supplementation with 4%, 8%, and 12% corn resistant starch (RS), respectively.

²⁾Orthogonal polynomial contrast was used to determine linear and quadratic effects of increasing concentrations of resistant starch in CS and RS diets.

^a–dMeans in a row without a common superscript letter significantly differ (p<0.05).