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. 2020 Oct 26;14:538404. doi: 10.3389/fnins.2020.538404

FIGURE 10.

FIGURE 10

(A) Photograph (40×) shows immunohistochemical expression of inducible nitric oxide synthase (iNOS) in middle cerebral artery occlusion (MCAO) model in control (C), ischemic (I), and treatment groups of nanosilymarin formulations NS1 (10 μg/kg b.wt), NS2 (100 μg/kg b.wt), NS3 (1,000 μg/kg b.wt), and free silymarin (Sil) (100 mg/kg b.wt). The control (C) group showed almost no expression for the iNOS marker, whereas the ischemic (I) group shows maximum expression. Treatments NS1 and NS2 showed moderate to mild expression but less than free silymarin; NS3 shows minimum expression. (B) Quantification of iNOS expression intensity as optical density (OD). The experiment was conducted for n = 3 data sets, and results are shown as mean ± SEM. Results on comparison with the control group showed a significant difference (###p < 0.001). Results obtained showed significant difference from the ischemic group (∗∗∗p < 0.001 and ∗p < 0.05).