FIGURE 4.

Influence of Cos on mitochondrial membrane potential in 769-P cells. (A) 769-P cells exposed to Cos (40 μM) for 24 h and observed under fluorescent microscope after JC-1 staining. Untreated cells served as control. Red fluorescence = normal Δψm; green fluorescence = damaged Δψm. (B) Δψm after Cos exposure and JC-1 treatment, as determined flow cytometrically, showing that Δψm-depolarized cells increased by about 26.2 and 61.3% at 12 and 24 h, respectively. All results are presented as mean ± SD (n = 3). *p < 0.05; **p < 0.01, vs the control.