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. 2020 Oct 26;10:582273. doi: 10.3389/fonc.2020.582273

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Copyright © 2020 Fu, Wu, Cheng, Gao, Zhang, Ge, Zhou and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cos-mediated 769-P cell apoptosis was mediated via mitochondrial route. Bcl-2 mRNA (A) and (B) Bax mRNA levels, as determined with qRT-PCR. (C) Bax and Bcl-2 proteins. (D) Bax/Bcl-2 ratio. (E) Bax and cyt c in 769-P cells. All results are presented as mean ± SD (n = 3). *p < 0.05; **p < 0.01, vs the control. (F) Cytosolic proteins were isolated and analyzed by Western blot to measure the localization of Bax and cyt c.