FIGURE 9.

Cos-induced apoptosis and autophagy were associated with JNK activation in 769-P cells. Western-blotting results on protein expressions of ERK1/2 and p-ERK1/2 (A), p38 and p-p38 (B), and JNK and p-JNK (C). Phosphorylated proteins in each immunoblot were normalized to total protein content of the respective protein. (D) Results CCK8 assay on 769-P cells pretreated with MEK1/2 inhibitor PD98059 (10 μM), JNK inhibitor SP600125 (10 μM), or the p38 inhibitor SB203580 (10 μM) for 2 h prior to treatment with or without Cos (20 μM), for 24 h. (E) Apoptosis of 769-P cells pretreated with JNK inhibitor SP600125 (10 μM) for 2 h prior to treatment with or without Cos (20 μM). (F) Expression levels of autophagy-related proteins, as assayed using Western blot. All results are presented as mean ± SD (n = 3). *p < 0.05; **p < 0.01, vs the control.