Table I.
Polymerase chain reaction primer sequences.
| Gene | GenBank accession number | Forward primer | Reverse primer | Primer efficiency, % |
|---|---|---|---|---|
| Actin | NM_007393.5 | 5’-GGCTGTATTCCCCTCCATCG-3’ | 5’-CCAGTTGGTAACAATGCCATGT-3’ | 106.965 |
| Tgfb1 | NM_011577.2 | 5’-CACCTGCAAGACCATCGACA-3’ | 5’-CATAGTAGTCCGCTTCGGGC-3’ | 101.098 |
| Alk5 | NM_009370.3 | 5’- CCTCGAGACAGGCCATTTGT-3’ | 5’- GCCAGCTGACTGCTTTTCTG-3’ | 113.534 |
| Col1 | NM_007742.4 | 5’-TTCTCCTGGCAAAGACGGACTCAA-3’ | 5’-AGGAAGCTGAAGTCATAACCGCCA-3’ | 113.257 |
| Pai1 | NM_008871.2 | 5’-TCTGGGAAAGGGTTCACTTTACC-3’ | 5’-GACACGCCATAGGGAGAGAAG-3’ | 107.895 |
| Alp | NM_007431.3 | 5’-ATCGGAACAACCTGACTGACCCTT-3’ | 5’-ACCCTCATGATGTCCGTGGTCAAT-3’ | 119.631 |
| Bsp1 | NM_001204203.1 | 5’-TGCCCTCTGATCAGGACAAC-3’ | 5’-ATCCGACTGATCGGCACTCT-3’ | 113.257 |
Alk5, activin receptor-like kinase-5; Alp, alkaline phosphatase; Bsp1, bone sialoprotein I; Col1, collagen type I; Pai1, PAI-1; Tgfb1, transforming growth factor-beta 1.