Fig. 7.

RT-PCR experiments to quantify FOXP3 and RORγt expression to further confirm the T cell phenotype. A significant increase in FOXP3 expression can be seen in Gal1 and Gal1–Gal2. Interestingly, a significant increase in RORγt production can be noted in Gal1–Gal2 and Gal1, whereas a reduction is observed in Man1–Gal2 indicating a possible presence of Th17 and Tregs in the population for Gal1–Gal2 and Gal1 and the absence of Th17 in Man1–Gal2 condition. Data are shown as mean ± SD of triplicates of three independent donors.