Table 2.
Glycosyl linkage analysis of hydrated, PBS-washed, SDS-extracted and alkali-extracted mycelium of S. commune. Abundances are given by peak area in percent calculated from the EI detector response. Preparational artefacts are grouped in ‘others’. They are observed in higher amounts for the alkali treated sample since we used less material for the analysis here.
Carbohydrate Linkage | Hydrated area % | PBS area % | SDS area % | KOH area % |
---|---|---|---|---|
hexp(1→ | 10.4 | 0.6 | 0.6 | 0.8 |
→3)hexp(1→ | 22.9 | 12.9/4.3 | 10.2/3.39 | 4.8 |
→4)hexp(1→ | 13.6 | X | X | X |
→6)hexp(1→ | 6.3/0.4 | 8.3/1.8 | 6.0 | 4.1 |
→3,4)hexp(1→ | 9.7 | 4.5 | 1.2/7.3 | 2.5 |
→2,3)hexp(1→ | 3.1 | 1.1 | 2.8 | 1.0 |
→2,4)hexp(1→ | X | 0.5 | X | 0.8 |
→4,6)hexp(1→ | 4.3 | 2.0 | 2.8 | 1.0 |
→3,6)hexp(1→ | 8.7 | 12.5/1.5/0.5 | 16.2/3.2 | 5.3 |
→2,3,4)hexp(1→ | 1.30 | X | X | X |
→3,4,6)hexp(1→ | 3.4 | 3.1 | 0.6/8.5 | 3.2 |
→2,3,6)hexp(1→ | 0.5 | 1.0 | 0.3/2.7 | X |
→2,4,6)hexp(1→ | X | X | 0.4 | X |
→2,3,4,6)hexp(1→ | 0.3 | X | 1.6 | 0.9 |
→4)hexpNAc(1→ | 3.4 | 20.2 | 14.3 | 22.6 |
→3)hexpNAc(1→ | X | X | X | X |
→3,4)hexpNAc(1→ | 0.3 | 2.6 | 2.0 | 2.8 |
→4,6)hexpNAc(1→ | 0.6 | 0.7/8.1 | 7.3 | 9.1 |
→3,4,6)hexpNAc(1→ | X | 0.8 | 0.9 | 0.9 |
other | 10.9 | 13.1 | 8.0 | 60.0 |