Figure 1.

Reconstruction of the biotinylated dextran amine (BDA) injection sites. (A) Reconstruction of the animals injected with BDA in the premotor cortex and (B) for the animals injected in the primary motor cortex (M1). The microphotographs show examples of one histological frontal section, from which the extent of BDA uptake (red lines) are transposed to the 3D brain (red line). Next to each frontal section, the insets show a photomicrograph at high magnification illustrating BDA-labeled cortical neurons in the vicinity of the core of the injection site. Scale bar = 10 mm for the 3D brains, 2 mm for the frontal sections, and 50 μm for the magnifications microphotographs. Modified from Fregosi et al. (2017); Fregosi et al. (2018).