Figure 2.

Sheep feces sample matrix trials 1 (A) and 2 (B): growth curves of Salmonella Typhimurium 37 (ST37) in pre-enrichment broth following low-, medium-, and high-dose inoculation. PCR data points derived from duplicate DNA extractions analyzed in duplicate reactions (n = 4 data points/concentration). Accurate ST37 quantitative culture data could not be obtained because of the growth of coliform colonies and so were excluded. Dashed horizontal line at 2.00 = theoretical limit of detection. BPW = buffered peptone water; MSCB = mannitol–selenite–cystine broth.