Table 2.
Antibody panel used for flow cytometric immunophenotyping of peripheral blood and mediastinal mass samples from a dog with thymoma-associated lymphocytosis.
| Antigen | Fluorophore | Clone | Manufacturer |
|---|---|---|---|
| CD34 | PE | 1H6 | eBioscience (Thermo Fisher Scientific) |
| CD45 | FITC | YKIX716.13 | eBioscience (Thermo Fisher Scientific) |
| CD5 | PerCP-eFluor 710 | YKIX322.3 | eBioscience (Thermo Fisher Scientific) |
| CD4 | Pacific Blue | YKIX302.9 | Bio-Rad |
| CD8 | AlexaFluor 700 | YCATE55.9 | Bio-Rad |
| CD21 | AlexaFluor 647 | CA2.1D6 | Bio-Rad |
| CD14 | APC-H7 | M5E2 | BD Biosciences |
| CD11b | PE-Dazzle 594 | M1/70 | Bio-Legend |
APC = allophycocyanin; CD = cluster of differentiation; FITC = fluorescein isothiocyanate; PE = phycoerythrin; PerCP = peridinin–chlorophyll–protein.