Table 1.
Interface residues of the homotypic TMD dataset.
| # | Protein (acca) [ref] | TMD sequenceb |
|---|---|---|
| ETRA TMDs | ||
| 1 | Ire1 (O75460)f | ATIILSTFLLIGWVAFIITY |
| 2 | ATP1B1 (P05026)[38]f | LLFYVIFYGCLAGIFIGTIQVMLLTI |
| 3 | PTPRG (P23470)[39] | IIPLIVVSALTFVCLILLIAVLV |
| 4 | Tie1 (P35590)[37] | LILAVVGSVSATCLTILAALLTLV |
| 5 | DDR1 (Q08345)[37] | ILIGCLVAIILLLLLIIALML |
| 6 | PTPRO (Q16827)[39] | VVVISVLAILSTLLIGLLLVTLIIL |
| 7 | Armcx6 (Q7L4S7)[35] | REVGWMAAGLMIGAGACYCV |
| 8 | PTPRU (Q92729)[39] | LILGICAGGLAVLILLLGAIIVII |
| 9 | Siglec7 (Q9Y286)[35] | VLLGAVGGAGATALVFLSFC |
| 10 | GpA (P02724) [7] | LIIFGVMAGVIGTIL |
| 11 | ErbB2 (P04626) [7], [56] | LTSIISAVVGILLVVVLGVVFGIL |
| 12 | ITGB3 (P05106) [57] | VLLSVMGAILLIGLAALLI |
| 13 | ITGA2B (P08514) [58]f | WVLVGVLGGLLLLTILVLAMW |
| 14 | FtsB (P0A6S5) [59] | TLLLLAILVWLQYSLWF |
| 15 | GP1BB (P13224) [60] | GALAAQLALLGLGLLHALLL |
| 16 | MPZ (P25189) [61] | YGVVLGAVIGGVLGVVLLLLLLFYVV |
| 17 | PTPRJ (Q12913) [39] | ICGAVFGCIFGALVIVTVGG |
| 18 | BNIP3 (Q12983) [62]f | LLSHLLAIGLGIYIG |
| 19 | QSOX2 (Q6ZRP7) [63] | CVVLYVASSLFLMVMY |
| 20 | ADCK3 (Q8NI60) [64] | LANFGGLAVGLGFGALA |
| 21 | NS4A (Q99IB8) [65] | TWVLAGGVLAAVAAYCLAT |
| NMR TMDs | ||
| 22 | CD3ζζ (P20963, 2hac)f | LCYLLDGILFIYGVILTALFL |
| 23 | EphA1 (P21709, 2k1k) | IVAVIFGLLLGAALLLGILVF |
| 24 | TYROBP (O43914, 2l34) | LAGIVMGDLVLTVLIALAVYFL |
| 25 | APP (P05067, 2loh) | AIIGLMVGGVVIATVIVITLVML |
| 26 | PDGFRB (P09619, 2l6w) | VVVISAILALVVLTIISLIILIMLW |
| 27 | FGFR3 (P22607, 2lzl) | VYAGILSYGVGFFLFLLVVAAVTLC |
| 28 | TLR3 (O15455, 2mk9)f | FFMINTSILLI̲FIFIVLL |
| 29 | DR5 (O14763, 6nhw) | SGIIIGVTVAAVVLIVAVFVCKSLL |
| X-ray TMDs | ||
| 30 | KvAP (P01837, 1orqC4) | GKVIGIAVMLTGISALTLLIGTVSNMFQ |
| 31 | Bacteriorhodopsin (Q8YSC4, 1xioA4) | GFLMSTQIVVITSGLIADL |
| 32 | PSII-M (Q8DHA7, 2axtM1) d | ATALFVLVPSVFLIILYV |
| 33 | Mgst1 (P08011, 2h8aA2) | HLNDLENIVPFLGIGLLYSL |
| 34 | Wza (Q9X4B7, 2j58A1)fd | SQLVPTISGVHDMTETVRYI |
| 35 | p2X purinoceptor (Q6NYR1, 3h9vA2) | KFNIIPTLLNIGAGLALLGLVNVICDWIV |
| 36 | GluCl α (G5EBR3, 3rifA2) | IPARVTLGVTTLLTMTAQSAGIN |
| 37 | KCNJ12 (F1NHE9, 3spcA2) | PLAVFMVVVQSIVGCIIDSFMIGAIMAKM |
| 38 | fn ATPase F0 c-ring (Q8RGD7, 3zk1A1)f | LGCSAVGAGLAMIAGLGPGIGEG |
| 39 | CRCM1 (Q9U6B8, 4hksA1) | SWTSALLSGFAMVAMVE |
| 40 | CorA (Q9WZ31, 4i0uA1) | TIIATIFMPLTFIAGIYGMNF |
| 41 | pntAB (Q72GR9, 4o9pC1) | WSALYI̲FVL̲T̲AF̲L̲GYE̲L̲ |
| 42 | AbgT (Q0VR69, 4r0cA7) | ITAMEVTMASMAGYLVLMFF̲AAQFVAWF |
| 43 | TspO (Q81BL7, 4ryiA2)f | PGMTIGMIWAVLFGLIALSVA |
| 44 | TMEM16 (C7Z7K1, 4wisA1) | LKAWGLLLSILFAEHFYLVVQLAVR |
| 45 | Trpv1 (O35433, 5irzD6)e | KAVFIILLLAYVILTYILLLNMLIALM |
| 46 | CRCB TM1 (Q7VYU0, 5nkqA1)f | F̲IAI̲G̲IGAT̲LGAW̲LRW̲VLG |
| 47 | CRCB TM3 (Q7VYU1, 5nkqA3) | AAVTGFLGGLTTFSTFSAETV |
| 48 | PC2 (Q13563, 5t4dA6)e | RVLGPIYFTTFVFFMFFILLNMFLAIIN |
| 49 | BCNG-1 (O60741, 5u6oA6)e | ITMLSMIVGATCYAMFVGHATALI |
| 50 | NadC (Q9KNE0, 5uldA9) | WKEIQKTADWGILLLFGGGLCL |
aAccession number (acc) from the UniProt database. The X-ray identification code (e.g. 1orqC4) consists of the PDB accession (e.g. 1orq), the protein chain (e.g. C), and the TMD number in the protein (e.g. 4).
bHomotypic interface residues in the TMD sequences are underlined.
cBold text indicates new interfaces identified in the current study. In these cases, the reference indicates the ETRA study in which the TMD was first tested, rather than the source of the mutagenesis data.
dTMDs in the X-ray dataset derived from bitopic proteins.
eTMD investigated by high-resolution electron microscopy.
fTMDs included in the blind test data for THOIPA validation.