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. 2020 Oct 22;11:569876. doi: 10.3389/fpls.2020.569876

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Copyright © 2020 Berg, Hermans, Beenders, Lou, Vriezen, Visser, Bai and Schouten.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Functional characterization of CsLRK10L genes. (A) Multiple protein sequence alignment of predicted proteins encoded by CsLRK10L1 and CsLRK10L2. A graph indicates conservation per amino acid. Pfam domains as identified by InterProScan v5.27 are indicated by arrows. (B) Alleles of CsLRK10L1 and CsLRK10L2 cloned from cucumber genotypes HS279 and NIL DM4.1.1/.2 were transiently expressed in Nicotiana benthamiana leaves by agro-infiltration (infiltrated leaf areas are indicated). Empty A. tumefaciens cultures were used as a negative control. CsLRK10L2 cloned from NIL DM4.1.1/.2 consistently triggered a necrotic reaction in >20 individual plants. In order to visualize necrotic tissue, leaves were stained using trypan blue.