Figure 2.

Monocyte MP response. (A) Gating schematic for live monocytes and indicated markers. (B) Expression of scavenger receptor CD163 on monocytes (n = 10) with media (white bars) PLGA MPs (gray bars) or full dMP (black bars). (C) Intracellular expression of IDO (n = 3) after incubation with PLGA or dMP (indicated as in (B)) shows increased frequency of IDO expression in media alone with dMP (left), to the level of the IDO-inducing stimulus LPS (right). (D) PD-L1 frequency (n = 6) increased significantly with dMP in the presence of LPS. Batch effects considered in analysis. (*p < 0.05, **p < 0.01, ***p < 0.001 by ANOVA with Tukey’s multiple comparison test).