Figure 7.

Assessment of antigen-specific T cell dMP response. (A) Schematic for generation of CD4 and CD8 T cell avatars via lentiviral transduction and coculture with dMP-treated HLA-selected APCs. (B) Results from target-killing assay for CD8 T cell avatars against the βlox5 beta cell line, with results represented as the ratio of dead labeled target cells to GFP⁺ T cells. (C) Expression of the cytotoxic effector protein Granzyme B in gated GFP⁺ T cells. (D) Proliferation in mock transduced T cells with allogeneic DC stimulus (left) was lower in presence of dMP, while antigen induced proliferation (right) was significantly controlled by dMP-APCs. (E, F) Analysis of intracellular staining of transcription factors associated with Treg (FOXP3) and T cell exhaustion (Eomes) showing difference of expression frequency with dMP pretreatment (E, % positive of gated GFP⁺ TCR⁺ cells − % positive in mock-transduced T cells) and difference of expression intensity (F, gMFI of gated GFP⁺ TCR⁺ cells – gMFI in mock-transduced T cells). (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 by ANOVA with Tukey’s multiple comparison test).