Table 1.

Encapsulation efficiency and effective microparticle dose.

Encapsulate	Input(ng/mg PLGA)	Encapsulation Efficiency (% ± SD)	MP mass in vitro (mg)	24 h dose (ng/mL)	48h dose (ng/mL)
rhTGF-β1	50	63.1 ± 6.6	5	67.1	88.5
rhGM-CSF	80	53.2 ± 7.3	5	34.6	76.8
Vitamin D3	100	73.4 ± 8.2	0.05	0.85	1.1

Encapsulation efficiencies were calculated via spectrophotometric analysis of microparticle (MP) batches. In vitro dosing was calculated based on the delivered MP mass and % release at defined time points.