Table 1. Structures and associated in vitro testing results for N-ethyl substituted analogs of compound 1.

Compound number	Ar	T. brucei a EC50 (nM)	CRL-8155 b CC50 (nM)	HepG2 c CC50 (nM)
2		40	>50 000	>50 000
3		>2000	>50 000	>50 000
4		31 ± 4 (n = 2)	45 000	>50 000
5		26 ± 3 (n = 4)	41 000	40 600
6		210	>50 000	>50 000
7		69	27 100	45 300
8		1600	38 200	43 700
9		660	31 600	31 300
10		220	20 900	29 800
11		110	>50 000	>50 000
12		>2000	>50 000	>50 000
13		17 ± 3 (n = 2)	>50 000	>50 000
14		390	>50 000	>50 000
15		1.6 ± 0.2 (n = 5)	>50 000	>50 000
16		3.4 ± 0.1 (n = 3)	>50 000	>50 000

^aControl for T brucei EC₅₀ assay average ± SEM: pentamidine (1.7 ± 0.2 nM; n = 14). Compounds with initial EC₅₀ < 30 nM were tested more than once and their EC₅₀s are shown as average ± SEM (number of tests).

^bControl for CRL-8155 CC₅₀ assay average ± SEM: quinacrine (7.0 ± 3.7 μM; n = 3).

^cControl for HepG2 CC₅₀ assay average ± SEM: quinacrine (7.6 ± 0.9 μM; n = 5).