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. 2020 Sep 25;2(1):vdaa127. doi: 10.1093/noajnl/vdaa127

Figure 4.

Figure 4.

TAMs survive the transplantations and are M2-polarized independently of tumor progression. (A) Schematic depicting the experimental approach for lineage-tracing of stromal cells. (B) Tomato+ (red) cells survive the transplantation and can be found in secondary tumors. Scale bar: 300 µm; 50 µm z-stack. Right: magnification of boxed area, scale bar 100 µm. (C) Tomato+ surviving cells are F4/80+. Scale bar: 100 µm. N = 4 tumors. (D) Experimental approach for macrophage-specific lineage-tracing. (E) Cx3cr1-GFP+ surviving cells in the secondary tumor. Scale bar: 150 µm; 50 µm z-stack. Right: magnification of boxed area, scale bar 50 µm. (F) Flow cytometry sort of surviving and host TAMs in a 4 + 4 weeks secondary tumor. N = 7 tumors. (G) Characterization of surviving TAMs in secondary tumors by immunofluorescent staining. Scale bar: 100 µm; 50 µm z-stack. ****P < .0001 (H) Proliferation of surviving cells in transplanted tumors. Scale bar: 50 µm. Data shown: mean + SEM (F–H). Each dot represents 1 tumor (G and H).