Figure 6.

Versican and HAPLN1 distribution. Confocal microscopy and 3D image analysis of HLFs in 10% FBS stained for HAPLN1 (red) and versican (green). (A) en face view in an optical section midway through the plane of the nucleus, showing HAPLN1 and versican are present in the nucleus (arrow). (B) oblique view indicating the plane of the image stack for the horizontal slice shown in (C). Arrows indicate the closely juxtaposed cytoplasmic deposits that are positive for versican and HAPLN1. (D) a maximum side view projection of the entire field with the cell removed to show apical and basal pericellular matrix. Note how versican dominates in the matrix above the cells (arrowhead), while HAPLN1 has relatively more signal underneath the cells (arrow). Nuclei were stained with DAPI. Scale is indicated. Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; FBS, fetal bovine serum; HAPLN1, hyaluronan and proteoglycan link protein 1; HLF, human lung fibroblasts.