Fig. 3. (A) Concentration-dependent effects of 15–20 on [3H]NMS (c = 0.2 nM) equilibrium M2R binding determined at intact CHO–hM2R cells. (B) Radioligand displacement curves obtained from competition binding experiments with [3H]NMS (0.2 nM (M1R, M2R, M3R), 0.1 nM (M4R) or 0.3 nM (M5R)) and compound 20 performed at intact CHO–hMxR cells (x = 1–5). M2R data are the same as in A. Data in A and B represent mean values ± SEM from two (20, M5R) or at least three independent experiments (each performed in triplicate).
