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. 2020 Nov 3;13:847–857. doi: 10.2147/JIR.S280091

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© 2020 Xu et al.

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JNK activator Anisomycin effected the related protein of apoptosis and autophagy caused by S. aureus infection in RAW264.7 cells. RAW264.7 cells treated with a JNK agonist anisomycin for 30 min, then treated with MG (25, 50, 100 μM) for 1h and infected with S. aureus (MOI:100) for 4 h. (A) Expressions of JNK, P-JNK, BAX, Beclin-1 and LC3 protein were analysed by Western blotting in RAW264.7 cells. Gels were representative of three independent experiments. (B) The graph represents a quantitative analysis of the band intensity. *P˂0.05 compared with SA group. (C) The graph represents a quantitative analysis of the band intensity.