Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 31;295(45):15342–15365. doi: 10.1074/jbc.RA120.014467

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Diering et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

Figure 7. — Oxidation-mediated effects of NCA on PKA and PP2A-C translocation. Following incubation of ARVMs with vehicle (control), NCA (100 µmol/liter, 30 min), or ISO (10 nmol/liter, 10 min), cells were harvested under NR or R conditions and separated into input Triton-soluble and Triton-insoluble fractions. cTnI was employed as the Triton-insoluble fraction marker protein. The content of PKA-RI, PKA-C, B56α, and PP2A-C in input lysates and in the Triton-insoluble fractions from both harvesting conditions was compared in 3 independent experiments. Signals obtained from Triton-insoluble samples were normalized to the related inputs and are presented as fold-change of vehicle control (R) in the scatter plots. **, p < 0.01; ***, p < 0.001 for comparison with the corresponding vehicle control; #, p < 0.05 for comparison between similar treatments from different harvesting conditions by two-way ANOVA with Bonferroni post-test (F and P values): a, PKA-RI, interaction, F = 4.24, p = 0.0403; treatment, F = 6.61, p = 0.0116; harvesting conditions, F = 3.91, p = 0.0714; b, PKA-C, interaction, F = 6.30, p = 0.0135; treatment, F = 11.03, p = 0.0019; harvesting conditions, F = 0.2196, p = 0.6477; c, B56α, interaction, F = 2.93, p = 0.0917; treatment,, F = 9.66, p = 0.0032; harvesting conditions, F = 5.89, p = 0.0320; d, PP2A-C, interaction, F = 4.23, p = 0.0406; treatment, F = 8.35, p = 0.0053; harvesting conditions, F = 9.86, p = 0.0085).