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. 2020 Aug 31;295(45):15292–15306. doi: 10.1074/jbc.RA120.013634

Figure 9.

Figure 9.

Combined inhibition of PI3K, MTOR, and ACK1 partially blocks IGF1 effect on FOXO1–GFP nuclear efflux. A and C, time course experiment and N/C bar graph (not normalized) at 90-min time point using an ACK1 inhibitor (AIM100), a PI3K inhibitor (BKM120), and IGF1. Control (A, solid black line; C, black circles) has no added compounds. PI3K inhibition and ACK1 inhibition (A, dashed lines; C, squares). IGF1 was added at 30 min (A, red lines; C, red circles and squares). B and D, time course experiment and N/C bar graph (not normalized) at the 90-min time point using ACK1 inhibitor (AIM100), PI3K inhibitor (BKM120), MTOR inhibitor (Torin1), and IGF1. Control (B, solid black line; D, black circles) has no added compounds. PI3K inhibition, ACK1 inhibition, and MTOR inhibition (B, dashed lines; D, squares) are shown. IGF1 was added at 30 min (B, red lines; D, red circles and squares). NS, not significant.