Figure 4.
Cleavage of factor X variants by intrinsic Xase. Factor X variants (5 μm) were incubated with 50 nm IXa, 100 nm VIIIa, and 50 μm PCPS at 25 °C for 30 min. The products were separated by SDS-PAGE without (lanes 2–5) or after disulfide bond reduction (lanes 7–10), and bands were visualized by staining with Coomassie Brilliant Blue. Lane 1, molecular weight markers; lanes 2 and 7, uncleaved XWT; lanes 3 and 8, activated XWT; lanes 4 and 9, unactivated XR15Q; lanes 5 and 10, activated XR15Q. HC, heavy chain; LC, light chain. Molecular weights × 103 for the standards are listed in the left margin.