. 2020 Sep 29;12(10):1101. doi: 10.3390/v12101101

Table 3.

Sequence of primers and PCR parameters used in this study.

Name	Targeted Virus or Gene	Sequence	Reference	[Primers] (nM)	[MgCl₂] (mM)	Annealing Temperature (°C)	Size of Amplification Product
CMV1-F	CMV ^a	5′-GTA GAC ATC TGT GAC GCG A-3′	[28]	400	1.5	55	540 bp
CMV1-R	CMV ^a	5′-GCG CGA AAC AAG CTT CTT ATC-3′	[28]	400	1.5	55	540 bp
Seco-1F	DMaV ^a	5′ AAC TCC WTC WGG WTT YGC TYT GAC 3′	this study	400	2	46	323 bp
Seco-1R	DMaV ^a	5′ CCC ACT TYC TYT TGA GAA AAT CAA 3′	this study	400	2	46	323 bp
DiosClos-F	YaV1 ^a	5′ CTC TTT AGG TTT CCC ATT TAT CA 3′	[13]	800	2	49	285 bp
DiosClos-R	YaV1 ^a	5′ TGG TTC TAC ATT ACT AGA CTA C 3′	[13]	800	2	49	285 bp
YMV1	YMV ^a	5′ TGC GGA ACT CRA AAG AAC 3′	[29]	200	3	53	196 bp
YMV2	YMV ^a	5′ TGC CAT CAA ATC CAA ACA 3′	[29]	200	3	53	196 bp
YMMV CP 2F	YMMV ^a	5′ GGC ACA CAT GCA AAT GAA AGC 3′	[30]	300	3	53	249 bp
YMMV UTR 1R	YMMV ^a	5′ CAC CAG TAG AGT GAA CAT AG 3′	[30]	300	3	53	249 bp
YamMac4F	Yam macluraviruses ^b	5′ CHG CAG CWA TYG GKM GTG 3′	this study	600	2	47	292 bp
YamMac5R	Yam macluraviruses ^b	5′ GGG TTG CTG AGC RTY GGA 3′	this study	600	2	47	292 bp
Potex-2RC	Potexviruses ^c	5′ AGC ATR GCN SCR TCY TG 3′	[31]	400	2	49	584 bp
Potex-5		5′ CAY CAR CAR GCM AAR GAY GA 3′
YamX-1F	Yam	5′ GAY ATT GGR GAY RTW YTI TTY WKG 3′
YamX-2F	Potexviruses ^b	5′ GRC ART CYC CAG ATT TYR AYH YRM A 3′
YamX-3F ^d		5′ CIC ART GGG TIA AGA AAR WKG A 3′	this study	300	2	43	255 bp
YamX-4R		5′ CAT IAC AGT TTS TTG CAT RAA IG 3′
YamX-5R ^d		5′ GRT CRA AIG CIG TRA ART CAT TIG C 3′
BadnaFP	Badnaviruses ^c	5′ ATG CCI TTY GGI ITI AAR AAY GCI CC 3′	[32]	200	3	49	579 bp
BadnaRP		5′ CCA YTT RCA IAC ISC ICC CCA ICC 3′
atpB1	atpB plastid gene	5′ GGT CCT ATG ATT TCC ACA C 3′	[33]	200	3	49	1462 pb
atpB2		5′ CTT CAA TTT GTT CTC CAC 3′

^a Specific detection primers; ^b Generic detection primers for yam viruses; ^c Generic detection primers; ^d Selected primers for detection of yam potexviruses.