Table 1.
In vitro feeding for the virus transmission study of Ixodes ricinus females infected with WNV lineage 1 during nymphal stage.
| FUs | Feeding Status | Engorged Ticks | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| D1 | D2 | D3 | D4 | D5 | D6 | D7 | D8 | D9 | D10 | D11 | D12 | D13 | D14 | D15 | ||
| FU1-1 | N | N | N | N | F | F | F | F | F | N | X | X | X | X | X | 0 |
| FU1-2 | N | N | N | N | N | N | N | N | N | X | X | X | X | X | X | 0 |
| FU1-3 | N | N | N | F | F | F | F | F | F | F | F | F | H | 1 a | ||
| FU1-4 | F | F | F | F | F | F | F | F | N | N | N | N | H | 1 a | ||
| FU2-1 | F | F | F | F | F | F | F | F | N | N | N | N | H | 1 b | ||
| FU2-2 | N | X | X | X | X | X | X | X | X | X | X | X | X | X | X | 0 |
| FU2-3 | N | N | F | F | F | F | N | F | F | F | F | F | F | N | H | 1 a |
| FU2-4 | N | N | N | F | F | F | F | F | F | F | X | X | X | X | X | 0 |
| FU2-5 | N | F | F | F | F | F | F | F | F | H | 1 b | |||||
| FU2-6 | F | N | X | X | X | X | X | X | X | X | X | X | X | X | X | 0 |
| FU3-1 | F | F | F | F | F | F | F | F | F | F | H | 1 a | ||||
| FU3-2 | N | N | N | N | N | F | F | F | F | F | N | N | H | 1 b | ||
| FU3-3 | N | N | N | N | N | N | F | N | N | X | X | X | X | X | X | 0 |
| FU3-4 | F | F | F | F | X | X | X | X | X | X | X | X | X | X | X | 0 |
| FU3-5 | N | N | F | F | F | X | X | X | X | X | X | X | X | X | X | 0 |
| FU3-6 | F | F | F | X | X | X | X | X | X | X | X | X | X | X | X | 0 |
| FU3-7 | N | N | N | F | F | F | F | F | F | X | X | X | X | X | X | 0 |
| FU4-1 | F | F | F | X | X | X | X | X | X | X | X | X | X | X | X | 0 |
| FU4-2 | F | F | F | F | F | F | F | N | N | N | H | 1 a | ||||
| FU4-3 | N | N | N | N | N | N | N | N | N | X | X | X | X | X | X | 0 |
| FU4-4 | N | N | N | F | F | N | X | X | X | X | X | X | X | X | X | 0 |
| FU4-5 | N | N | N | N | X | X | X | X | X | X | X | X | X | X | X | 0 |
| FU4-6 | N | N | F | F | F | F | F | F | F | F | H | 1 | ||||
| Total | 8 | 8 | 11 | 13 | 13 | 12 | 12 | 10 | 9 | 6 | 2 | 2 | 1 | 0 | 0 | 9 |
| Neg. control * | 3 | 3 | 3 | 4 | 4 | 5 | 4 | 5 | 5 | 5 | 3 | 3 | 1 | 1 | H | 3 c |
FUs: feeding units containing one Ixodes ricinus female from the experimental group; D1–D15: days of in vitro feeding; N: not feeding; F: time point when female ticks were observed to be attached to the membrane; X: feeding cancelled; H: day when engorged ticks were harvested; * Negative control FU with 6 females; a Engorged ticks that died during incubation; b Females that laid eggs; c Two out of three females from the control group laid eggs.