Table 2.
Primary antibody list.
| Antigen | Antibody | Source | MW (kDa) | Dilution | Application |
|---|---|---|---|---|---|
| Notch-1 | Rabbit mAb ab52627 | Abcam | 125 | 1:500, 1:20, 1:100 | WB, IHC, IF |
| Numb | Rabbit mAb 2761S | Cell Signaling | 72, 74 | 1:500 | WB |
|
Notch-2 |
Goat pAb ab4147 Rabbit mAb 4530S |
Abcam Cell Signaling |
110 |
1:20, 1:100 1:500, 1:50, 1:50 |
IHC, IF WB, IHC, IF |
| K15 | Mouse mAb ab52816 | Abcam | 45 | 1:5000, 1:100 | WB, IHC |
| Hey1 Hes1 β-Actin |
Rabbit pAb ab22614 Mouse mAb ab119776 Rabbit mAb 11988S Mouse mAb A5441 |
Abcam Abcam Cell Signaling Sigma |
34 30 42 |
1:50 1:50 1:500 1:2000 |
IHC IHC, IF WB WB |
pAb, polyclonal antibody; mAb, monoclonal antibody; WB, western blot; IHC, immunohistochemistry; IF, immunofluorescence.