Figure 7.

(A) Schematic representation of the experimental setup. Human fibroblasts of healthy controls (n = 7) and PARK6 patients (n = 3) were either left untreated or incubated with FAC, DFO, and 22BP for 16 h, and the extracted RNA was analyzed by RT-qPCR. (B) Changes of mRNA expression for key iron homeostasis factors, and crucial downstream effects, which had been significantly dysregulated in previous mouse experiments. Expression levels were normalized against HPRT1 mRNA as a loading control. Mean values with SEM are shown, normalized to the untreated control condition. The statistical trends or levels of significance are illustrated by symbols T: 0.1 > p>0.05, * or #: p < 0.05, ** or ##: p < 0.01, **** or ####: p < 0.0001. Asterisks represent significant changes in control fibroblasts, treated versus untreated control, while hashtags refer to PARK6 patient cells, treated versus untreated. Genotype-dependent significant differences between patients and controls are illustrated by horizontal lines below asterisks.