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. 2020 Sep 28;9(10):929. doi: 10.3390/antiox9100929

Figure 1.

Figure 1

Calcein fluorescence intensity strongly depended on oxidant to cell count ratio. Red blood cell (RBC) suspension was incubated with tert-Butyl hydroperoxide (t-BOOH) at indicated concentrations for 1 h, then RBCs were stained with calcein-AM (5 µM, 40 min) and analyzed for calcein fluorescence intensity by flow cytometry at FL1 (logarithmic scale). (A) Representative histograms from five independent experiments. (B) Dependence of calcein fluorescence intensity in constant RBC count from t-BOOH concentrations. (C) Dependence of calcein fluorescence intensity in constant t-BOOH concentration from RBC count. (D) Exponential dependency between calcein fluorescence intensity and the ratio of oxidant concentration/cell count ([t-BOOH]/RBC). Data in (B,C) are presented as means ± SD, n = 5.