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. 2020 Sep 28;9(10):929. doi: 10.3390/antiox9100929

Figure 2.

Figure 2

Oxidative stress induced by t-BOOH-triggered RBC transformations and microvesicle formation. RBCs (0.5 × 109 cells/mL) were incubated at indicated concentrations of t-BOOH, A23187, and S-nitroso-L-cysteine (SNC) at the indicated times. Gate 1 represents control RBCs, gate 2—microparticles (MPs), gate 3—transformed RBCs, and gate 4—microvesicles (MVs). (A) Representative dot plots out of six independent experiments. (B) Quantification of presented data expressed as mean ± SD, n = 6. One-way ANOVA, Tamhane T2 (G1, G2 24 h, G4), or Tukey HSD post hoc (G2 3 h) were used where appropriate. * p < 0.05, ** p < 0.001 compared to control (t-BOOH 0mM, 3 h); # p < 0.05, ## p < 0.001 compared to control (t-BOOH 0mM, 24 h).