Figure 5.

Oxidative stress (OS)-induced decrease in osmotic fragility of RBCs. RBCs (0.5 × 10⁹ cells/mL) were incubated with the indicated substances for 1h, and then aliquots (10 µL, 10⁶ cells/mL final concentration) of samples were resuspended in HEPES buffer with EGTA to register light scattering intensity corresponding to control. Then, the osmolality was gradually reduced by H₂O supplementation, from 300 to 70 mOsm/kg H₂O, to maintain the RBC concentration, and the corresponding number of cells was added at each step of H₂O supplementation. (A) Representative osmotic hemolysis curves from the osmotic fragility test (OFT). (B) Quantification of percentage of hemolysis from osmotic fragility test calculated from six independent experiments. (C) Quantification of MCV during osmotic fragility test calculated from six independent experiments.