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. 2020 Sep 24;12(10):1071. doi: 10.3390/v12101071

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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IFAT targeting E2 in CHSE-214 cells: 7 dpt, 10X; (A) rSAV3; (B) rSAV3 E2_319Q; (C) rSAV3 E2_319A; (D) rSAV3 E1_35Q; (E) rSAV3 E1_35A; (F) rSAV3 E1_35Q E2_{319Q; and} (G) rSAV3 E1_35A E2_319A. Strong staining in cells transfected with the rSAV3 clone and the two constructs containing mutations in E2₃₁₉ only (A–C). Weak or no staining in cells transfected with constructs containing mutations in E1₃₅ (D–G).