Figure 5.

Salt stress tolerance of wild-type (WT) and transgenic Brachypodium sylvaticum plants expressing pSARK: ΔN. (A) Shoot dry weight of WT and two transgenic lines (SARK: ΔN-1-1 and SARK: ΔN-19-3) grown under well-watered (WW) and salt stress (SS) conditions during the first harvest. Plants were harvested for the first time at around 45 days old, right after being treated with 100mM NaCl solution for 1 week, and then 200mM NaCl solution for 1 week continuously. (B) Shoot dry weight of wild-type (WT) and two transgenic lines during the second harvest. The second harvest was performed at 25 days after the first harvest, during which all the plants were well irrigated with distilled water. Values are means and SE (n= 12 for SARK:ΔN and 48 for WT). Asterisks represent significant differences between transgenic lines and WT within a treatment by the Dunnet test (p < 0.05).