Figure 4.

Densitometry validation and quantification of final RNA-Seq cDNA libraries prior to Illumina sequencing, using an Agilent 2100 Bioanalyzer and the High Sensitivity DNA kit (Beckman Coulter, Brea, CA, USA). The outermost peaks represent a size marker. RNA-Seq cDNA libraries were prepared from two replicates of RNA extracted from barley grains: (a) control samples with high viability according to the path A; (b) long-term stored samples with low viability according to the path B; (c) long-term stored samples with low viability according to the path A; (d) electrophoresis run of the cDNA libraries by the Bioanalyzer. The green and red frames indicate correct and incorrect fragment size range in the libraries, respectively.