Table 1.
Sample parameters obtained by the automatic electrophoresis Bioanalyzer 2100 (Rc—control samples with high viability, Lv—long-term stored samples with low viability; each sample represented by two biological replicates).
| Samples | Rc 1 | Rc 2 | Lv 1 | Lv 2 | |
|---|---|---|---|---|---|
| RIN | 7.9 | 7.8 | 2.5 | 2.8 | |
| RNA Area | 1437.8 | 1837.6 | 1253.1 | 820.5 | |
| RNA Concentration [ng/μL] | 1127.0 | 1441.0 | 983.0 | 643.0 | |
| rRNA Ratio [28S/18S] | 1.4 | 1.4 | 0.6 | 0.7 | |
| 18S | Start size [nt] | 1609.0 | 1590.0 | 1644.0 | 1616.0 |
| End size [nt] | 2193.0 | 2196.0 | 1973.0 | 1951.0 | |
| Area [%] | 147.2 | 181.8 | 15.7 | 14.8 | |
| % of total area | 10.2 | 9.9 | 1.3 | 1.8 | |
| 28S | Start size [nt] | 2615.0 | 2588.0 | 2748.0 | 2653.0 |
| End size [nt] | 3555.0 | 3559.0 | 3432.0 | 3293.0 | |
| Area [%] | 213.3 | 257.2 | 9.5 | 9.7 | |
| % of total area | 14.8 | 14.0 | 0.8 | 1.2 |