Table 2.

RNA, mRNA and library concentration (Rc—control samples with high viability, Lv—long-term stored samples with low viability; each sample represented by two biological replicates, A —cDNA synthesis path for samples with RIN > 3, B— cDNA synthesis path for samples with low RIN < 3).

Sample ID	Rc 1A	Rc 2A	Lv 1B	Lv 2B	Lv 1A	Lv 2A
RNA concentration after isolation [ng/µL] *	2103.51	2148.00	1932.05	1256.31	1932.05	1256.31
RNA concentration after DNA removal [ng/µL]*	900.40	901.56	906.50	904.28	906.50	904.28
mRNA fraction concentration [ng/µL] *	126.72	124.56	90.61	62.88	90.61	62.88
library concentration [ng/µL] **	11.1	11.31	0.76	0.82	5.38	5.4

* measured by NanoDrop 1000, ** measured by Qubit.