Figure 2.

SG assembly mutants do not strikingly affect TDP-43 toxicity, foci formation or abundance. (A) BY4741 (WT) and indicated strains were transformed with pRB109 expressing TDP-43-YFP and examined for TDP-43 toxicity by serial dilution spotting assay. (B) The strains mentioned above were examined for the presence of TDP-43 foci. (C) Quantification of (B); number of TDP-43-YFP foci/cell. Data generated from 3 biological replicates with mean ± s.d shown. (D) Western analysis of above strains to assess steady-state TDP-43-YFP protein levels; quantification, based on two biological replicates, is normalized to GAPDH loading control.