Figure 3.

Exploratory and differential expression analyses of RNA-seq data. (A) Principal-component analysis of sample relationship. (B) Poisson distance plot of sample dissimilarities in terms of transcriptomic profiles. (C) Volcano plot of shrunken log₂ (fold change) and –log₁₀FDR of all tested genes between vehicle-control (CK) samples and samples treated with USP7797 at 48 h post treatment. Genes with|log₂(fold change)| > log₂(1.5) and FDR < 0.05 (significantly differentially expressed genes) are in red, genes with|log₂(fold change)| > log₂(1.5) but FDR ≥ 0.05 are in green, genes with|log₂(fold change)| ≤ log₂(1.5) but FDR < 0.05 are in blue, and the rest are in gray. (D) Heatmap showing significantly differentially expressed genes which are represented by red dots in (C).