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. 2020 Oct 2;12(10):1121. doi: 10.3390/v12101121

Figure 4.

Figure 4

Localization of A. thaliana DRB4 to TBSV replication complexes. Laser confocal microscopy on 35S:B2:GFP/N. benthamiana leaves inoculated with A. tumefaciens carrying the construct of interest then infected with TBSV. For each acquisition we show the GFP channel on the left (B2), the tRFP channel in the middle (construct of interest) and a merge of the two on the right. (A) Observation of tissues expressing 35S:tRFP. (B) Observation of tissues expressing 35S:DRB4:tRFP. The lower row of three acquisitions is shown to highlight the intraperoxisomal B2 foci (white arrowheads). For the sake of simplicity, the labeling was omitted on these but is identical to that in the upper row. (C) Observation of tissues expressing 35S:tRFP:NFD2. All acquisitions were performed with a 63× objective. Scale bars indicate 5–10 μm, as indicated. Lower magnification acquisitions from these tissues, along with the respective non-infected controls, can be found in Supplementary Figure S4.