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. 2020 Oct 2;12(10):1121. doi: 10.3390/v12101121

Figure 6.

Figure 6

Processing of TBSV dsRNA by DCL4, but not DCL2, in A. thaliana. (A) Northern blot analysis of high molecular weight RNA to detect TBSV in systemically infected rosette leaves, 9 dpi, of A. thaliana mutant lines. Quantification of signal intensity is provided. Each sample is a pool of 4–5 plants. (B) Northwestern blot to detect dsRNA in the samples described in (A). The black arrow indicates the dsRNA band observed upon genetic knock-out of DCL4. In (A,B) EtBr gel staining was used as loading control. (C) Northern blot analysis of low molecular weight RNA to detect TBSV-derived siRNA, using two separate probes. TBSV-mid detects siRNA derived from the 3′ end of the P92 ORF, while TBSV-P19 detects siRNA derived from the P19 ORF. As a loading control, the membrane was separately hybridized to a snU6-specific probe. In all the blots a non-infected control (n.i.) was included on the far left.