Figure 1.

Effect of KAT (Lysine acetyl transferase) inhibitors on the acetylation of histone H4 induced by doxorubicin. (A). Time course of the effect of doxorubicin on the phosphorylation of endogenous Tip60 in serine detected with anti-phosphoserine antibody, and the acetylation of histone H4 in K16 in cells deprived of serum (0.5%) for 48 h and arrested in G0/G1 before treatment with doxorubicin (Doxo). Histones were prepared by acidic extraction. The reference value corresponds to the starting time point (0 min). (B). Induction of H4K16ac by treatment with doxorubicin (10 µM) and the effect of MG149 (Tip60 inhibitor, 2 µM) and C646 (p300 inhibitor, 10 µM) added ten hours before doxorubicin addition. The box plots show the quantification of the fluorescence using the Image J program. ns: Not significant. *** p < 0.001.