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. 2020 Oct 15;12(10):2986. doi: 10.3390/cancers12102986

Figure 5.

Figure 5

VRK1 knockdown prevents chromatin accumulation of endogenous Tip60 induced by doxorubicin. (A). Effect of doxorubicin on the Tip60 nuclear protein detected by immunofluorescence. siCt: si-control; si-V1-02: si-VRK1-02. The fluorescence was quantified in fifty cells using the Image J program. *** p < 0.001. (B). Effect of doxorubicin on the endogenous Tip60 and its phosphorylation in the nucleoplasm and chromatin fractions determined in control or VRK1-depleted A549 cells. In all time course experiments, the reference value is the one corresponding to the starting time point (0 min). All experiments were repeated four times.