Table 1.
Method validation (linearity, recovery, and precision of method).
| Method linearity | ||||
| Slope a | 11.348 ± 0.026 | |||
| Intercept a | 13.514 ± 3.908 | |||
| Correlation coefficient (r) | 0.999 ± 0.000 | |||
| LOD [mg/kg] | 3.6 | |||
| LOQ [mg/kg] | 11.8 | |||
| Method recovery b | ||||
| Reference material | Reference value [mg/kg] | Determined value [mg/kg] | Recovery [%] | RSD [%] |
| muva-BU-1312 | 2337 ± 196 | 2395.6 ± 72.6 | 102.5 ± 0.0 | 3.0 |
| muva-BU-1311 | 2334 ± 211 | 2381.6 ± 66.6 | 102.0 ± 0.0 | 2.8 |
| muva-BU-1314 | 2276 ± 135 | 2307.7 ± 58.9 | 101.4 ± 0.0 | 2.6 |
| The amount of cholesterol standard spiked to the butter sample [mg/L] | Determined value [mg/kg] | Recovery [%] | RSD [%] | |
| 0 | 2367.0 ± 29.9 | - | - | |
| 500 | 2460.6 ± 39.0 | 103.9 ± 0.0 | 1.6 | |
| 1000 | 2475.4 ± 31.5 | 104.6 ± 0.0 | 1.3 | |
| 1500 | 2515.9 ± 52.7 | 106.3 ± 0.0 | 2.1 | |
| Method precision c | ||||
| Reference material | Repeatability | RSD [%] | Intermediate precision [RSD%] | Horrat |
| muva-BU-1312 | Day 1 Day 2 Day 3 |
2.5 2.6 2.2 |
1.8 | 0.01 |
| muva-BU-1311 | Day 1 Day 2 Day 3 |
3.9 2.3 0.6 |
0.9 | 0.01 |
| muva-BU-1314 | Day 1 Day 2 Day 3 |
1.4 3.5 1.9 |
0.8 | 0.01 |
The values are expressed as mean ± standard deviation, a n = 4, b n = 12, c n = 4; muva-BU-1312, sweet cream butter; muva-BU-1311, butter mild soured; muva-BU-1314, sweet cream butter salted; LOD, limit of detection; LOQ, limit of quantification; RSD, relative standard deviation.