Table 2.

The ruggedness of proposed method.

Dimension of Column(Zorbax Eclipse Plus C18)	Retention Time [min]	Slope	Intercept	LOD [mg/kg]	LOQ [mg/kg]	Cholesterol Content [mg/kg] a
2.1 × 100 mm, 3.5 µm particle size	5.6	11.348	13.514	3.6	11.8	2367.0 ± 29.9
2.1 × 50 mm, 3.5 µm particle size	2.2	11.349	17.320	3.0	10.0	2360.4 ± 27.9
4.6 × 50 mm, 3.5 µm particle size	4.4	5.713	30.071	5.5	18.4	2289.3 ± 53.8
2.1 × 50 mm, 5.0 µm particle size	2.2	11.480	69.452	6.8	22.5	2417.5 ± 92.6

^a The values are expressed as mean ± standard deviation; LOD, limit of detection; LOQ, limit of quantification. Muva-BU-1311, butter mild soured was used for evaluation the method ruggedness.