Figure 5.

Expression of six selected genes over a time course. (A) Kinetics of yeast growth according to OD_600nm values. The cells were incubated with or without melatonin (5 µM) for 6 h, and two conditions (Mel, MelH) were subjected to 2 mM H₂O₂ treatment (0 h). (B) Expression of each gene of interest at different time points in the nonstressed and stressed conditions (Control, H), expressed as log₁₀(ratio). The ratio represents the 2^−ΔΔCt value corrected to the 2^−ΔΔCt value of the Control at 0 h. (C) Heat map of the effect of melatonin on the expression of the six genes during the time course analysis in nonstressed and stressed cells. The color corresponds to the log₁₀ of the ratio. The ratio represents the 2^−ΔΔCt value for the condition with melatonin corrected to the 2^−ΔΔCt value for the condition without melatonin. Genes are presented in rows, and the time points under both conditions are presented in columns. Exp, exponential phase; stat, stationary phase. High-intensity colors refer to values >0.35 (green) or < −0.35 (red) * significance level of < 0.05. Error bars represent the standard deviation, and the statistical analysis of B and C is presented in Table S3.